The Serbian star was involved in a moment of controversy during his second round win over Roberto Carballes Baena – a day after David Goffin was spat on by a fan.
And Djokovic was briefly jeered by those in attendance at Roland Garros early on in his 6-4, 6-1, 6-2 victory on Thursday following an altercation with a spectator.
In the first set when he was 5-4 ahead against his Spanish opponent, Djokovic complained to chair umpire Nico Helwerth that he had been heckled by a fan as he chased a drop shot.
As he made his complaints, loud boos were heard directed at the 37-year-old.
The heckler was told he would be removed from the arena if he carried on by the chair umpire.
And Djokovic has since revealed what happened during the incident.
“He shouted actually a few words during the rally,” said the world No. 1.
“I mean, when Carballes Baena made a drop-shot and as I was running, he was there in the first row – and he started from the beginning of the match.
“He went on several times, which was fine, he was supporting my opponent, but I thought that this was a bit of a hindrance as he was so close and then, you know, as I was about to hit the ball, he was shouting.
“So that’s what I asked the chair umpire, but he explained that he cannot call that a hindrance. That’s it. Very simple.
“Look, they are very passionate fans, you know. At times they are not easy.
“I have been having some love affairs with the fans here – and also some tough matches, some tough seasons. I have experienced both sides.
“Obviously you always want to be the one that they support or at least have kind of a neutral atmosphere on the court, but it’s not possible always for that to happen.”